A system containing chromogenic medium for selective enumeration of E. coli.
Detection and enumeration of Coliforms.
Each kit comprises
Plastic tube container hermetically sealed.
Plastic paddle (9cm2) coated with culture medium on both sides.
Plastic cap that may be taken apart from paddle.
Store at 15-25 °C. Protect from light.
Pkg.: 20 units in a box (4 X 5).
Instructions for Use and Interpretation of Results.
E. Coli side: Chromogenic powder 37.3 (CHROMagar – France);
Total Coliforms side: Violet Red Bile Lactose Agar 41.5 (DIFCO-USA / HIMEDIA-India);
Formula is adjusted for each batch.
E. Coli side: CHROMagar E. Coli is a chromogenic medium showing directly E. Coli on the base of specific chromogenic reactions. The medium can be used for monitoring food hygiene since E. Coli is an indicator of faecal contamination.
Total Coliforms side: VRBA is used for the detection and enumeration of coliform organisms in water, milk, dairy and other food products.
E. Coli side: Selective enumeration of E. Coli due to chromogenic reactions that detect the β glucoronidase activity specific for E. coli.
Total Coliforms side: Selection of lactose fermenter organisms (Coliforms) due to the presence in the medium lactose, natural red indicator, bile salts and crystal violet inhibit of gram-positive microorganisms especially Staphylococci.
1. Follow recommended procedures in the Instructions for Use.
2. If the product has been refrigerated, allow it to return to room temperature before sampling and incubate aerobically at the recommended conditions.
Interpretation of Results
E. Coli side: E. Coli grow in blue colonies.
- aerogenes grow in colorless colonies.
- aureus is inhibited.
Total Coliforms side: Gram negative organisms which rapidly ferment lactose form red colonies surrounded by a red-purple halo. Lactose nonfermenters and late lactose fermenters form pale colonies. Gram positive are inhibited.
For the dipping method: The sensitivity of the test is from 1×103 cfu/gr or ml.
Lactose fermenters grow in pale colonies after 24 hours.
Used contaminated test material should be handled by standard decontamination methods.