A system containing chromogenic media for the selective detection and enumeration of E. coli and the detection of Staphylococcus aureus.
Each kit comprises
Plastic tube container hermetically sealed.
Plastic paddle (9cm2) coated with culture medium on both sides.
Plastic cap that may be taken apart from paddle.
Store at 2-15 °C. Protect from light.
Pkg.: 20 units in a box (4 X 5).
Instructions for Use and Interpretation of Results.
E. Coli Side
Chromogenic mix (CHROMagar – France): 9.0
Peptone and East Extract: 8.3
PH: 5.8- 6.2
Staph Aureus Side
Chromogenic mix (CHROMagar – France): 2.5
Peptones and East Extract: 40.0
E. Coli – Selective enumeration of E. coli due to chromogenic reactions that detect the β glucoronidase activity specific for E. coli.
Staph Aureus – The formulation includes selected peptones that supply the nutrients. A mixture of chromogenic substrates releases an insoluble coloured compound when hydrolyzed by specific enzymes from S. aureus, leading to the growth of the microorganism in mauve colonies surrounded by a mate halo. The addition of selective agents to the medium inhibits the growth of most gram-negative bacteriae, S. epidermidis and yeasts.
1. Follow recommended procedures in the Instructions for Use.
2. If the product has been refrigerated, allow it to return to room temperature before sampling and incubate aerobically at the recommended conditions.
Used contaminated test material should be handled by standard decontamination methods.